Performance of multistage operation in affinity chromatography.
نویسندگان
چکیده
منابع مشابه
Preparation of Plasminogen by Affinity Chromatography
Background: Plasminogen is one of the compounds derived from human plasma. Activation of plasminogen produces plasmin. Plasmin is able to lyse fibrinogen, fibrin, and some other human plasma proteins. The aim of the present work was to study the separation of human plasminogen by affinity chromatography using gel lysine Sepharose. Materials and Methods: Normal human plasma was used as the...
متن کاملPreparation and Characterisation of Immunoadsorbents for High Performance Liquid Affinity Chromatography (HPLAC)
The efficacy of activation methods and coupling were studied in the context of performance in batch and fixed bed binding experiments utilizing cell culture fluids or blood plasma as feedstock. Conclusions were drawn regarding selection of solid phase according to pore size, rigidity, pH stability, Chemistry of derivation and activation, and gross concentration of immobilized ligand require...
متن کاملRecent advances in high-performance liquid affinity chromatography columns.
High-performance liquid affinity chromatography (h.p.1.a.c.) is the term applied to the application of h.p.1.c. to affinity chromatography (Ohlson et al., 1978). Until recently affinity chromatography supports were unsuitable for use in high-performace applications. It is only in the last 8 years that these problems have been overcome, resulting in a second generation of affinity systems. The a...
متن کاملpreparation of plasminogen by affinity chromatography
background: plasminogen is one of the compounds derived from human plasma. activation of plasminogen produces plasmin. plasmin is able to lyse fibrinogen, fibrin, and some other human plasma proteins. the aim of the present work was to study the separation of human plasminogen by affinity chromatography using gel lysine sepharose. materials and methods: normal human plasma was used as the start...
متن کاملAffinity Chromatography of Lectins
Affinity chromatography is a technique used to purify compounds, such as proteins, that have the ability to non-covalently and reversibly bind specific molecules, known as ligands. This method differs from the classical chromatography techniques in that the protein is purified on the basis of a unique biochemical property. In affinity chromatography, the ligand is covalently attached to a matri...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
سال: 1980
ISSN: 0021-9592,1881-1299
DOI: 10.1252/jcej.13.336